Canadian Forest Service Publications
Isozyme and protein patterns of endophytic and disease syndrome associated isolates of Melanconium apiocarpum and Melanconium marginale collected from alder. 1993. Shamoun, S.F.; Sieber, T.N. Mycotaxon Vol. XLIX: 151-166.
Available from: Pacific Forestry Centre
Catalog ID: 3321
CFS Availability: Order paper copy (free)
Isozyme and protein patterns of five of each Disease Syndrome Associated (DSA) isolates and Symptomless Endophytes (SE) of Melanconium apiocarpum as well as 10 DSA Melanconium marginale isolates from different species of alder were determined by polyacrylamide gel electrophoresis. Seven of the 13 enzyme systems tested produced positive activities: acid phosphatase, ß-glycosidase, malate dehydrogenase, phosphoglucomutase, ß-esterase, peroxidase and polyphenoloxidase, but only the latter three enzyme systems allowed differentiation between M. apiocarpum (SE and DSA) and M. marginale isolates. In addition, M. marginale isolates differed from M. apiocarpum in their non-SDS electrophoretic protein banding patterns, protein contents, yield of acetone powder and relative virulence on apple fruits. None of the methods used showed differences between SE and DSA isolates of M. apiocarpum. The use of _-esterase, peroxidase and polyphenoloxidase enzyme systems as potential biochemical markers is proposed as a taxonomic tool to differentiate M. apiocarpum (SE and DSA) from M. marginale isolates. These findings imply that DSA and SE isolates of M. apiocarpum are similar if not identical. Future research is directed toward manipulation of the SE isolates and their hosts (Alnus spp.), to promote them to pathogenic phase so that they can function as biological control agents (mycoherbicides) for control of alder on reforestation sites.
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