Canadian Forest Service Publications

Complete rRNA sequence, arrangement of tandem repeated units and phylogeny of Nosema fumiferanae from spruce budworm, Choristoneura fumiferana (Clemens). 2012. Kyei-Poku, G.; Gauthier,D.; van Frankenhuyzen, K. Journal of Eukaryotic Microbiology 59(1):93-96.

Year: 2012

Available from: Great Lakes Forestry Centre

Catalog ID: 33274

Language: English

CFS Availability: PDF (request by e-mail)

Abstract

We provide molecular systematics of a microporidian species, Nosema fumiferanae, one of the most common natural enemies of spruce budworm, Choristoneura fumiferana. The uncharacterized flanking region upstream of the large subunit (LSU) rRNA and the complete rRNA cistron of N. fumiferanae was 4,769 bp long. The organization of the rRNA gene was 5'-LSU rRNA-ITS-SSU rRNA-IGS-5S-3' and corresponded primarily to most insect (i.e. lepidopteran) Nosema species identified and classified to date. Phylogenetic analysis based on the complete rRNA cistron indicated that N. fumiferanae is closely related to Nosema plutellae and is correctly assigned to the "true" Nosema group. Suggestions were provided on a criterion to delineate the "true" Nosema from other microsporidian species.

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