Canadian Forest Service Publications

Activity of elements from the tobacco cryptic promoter, tCUP, in conifer tissues. 2003. Tian, L.; Wu, K.; Levasseur, C.; Ouellet, T.; Foster, E.; Latoszek-Green, M.; Sibbald, S.; Miki, B.; Séguin, A.; Brown, D.C.W. In Citro Cell. Dev. Biol.-Plant 39-193-202.

Year: 2003

Available from: Laurentian Forestry Centre

Catalog ID: 33681

Language: English

CFS Availability: PDF (request by e-mail)

Available from the Journal's Web site.
DOI: 10.1079/IVP2002365

† This site may require a fee.

Abstract

Cryptic gene regulatory elements are inactive at their native locations in the genome but are fully functional when positioned adjacent to genes in transgenic plants. Although they are widespread among diverse organismes, they have not yet been described in gymnosperms. tCUP is a strong constitutive cryptic promoter from tobacco that harbors transcriptional enhancers, a TATA-less core promoter, and a translational enhancer. tCUP is active in all conifer species tested including white spruce, white pine, Loblolly pine, larch, and Douglas fir. The tCUP enhancers are fully active in white spruce in combination with the tCUP or 35S core promoters. The core promoter is proportionally stronger in white spruce than in angiosperms. It is dependent on an Inr sequence at the transcription start site; however, a cryptic core promoter was found in the tCUP leader sequence that harbors another portential Inr site. The leader sequence is essential for full tCUP activity in white spruce and it can be combined with other promoters, such as the 35S promoter, te elevate activity further. Stable and constitutive expression of tCUP is demonstrated in transgenic trees of white spruce. tCUP provides an alternative system for the expression of cloned genes in conifer species.

Date modified: