Canadian Forest Service Publications

Towards the development of an autocontamination trap system to manage populations of emerald ash borer(Coleoptera: Buprestidae) with the native entomopathogenic fungus, Beauveria bassiana. 2012. Lyons, D.B.; Lavallée, R.; Kyei-Poku, G.; van Frankenhuyzen, K.; Johny,S.; Guertin, C.;Francese, J. A.; Jones, G.C.; Blais, M. Journal of Economic Entomology. 105(6):1929-1939.

Year: 2012

Issued by: Great Lakes Forestry Centre

Catalog ID: 34261

Language: English

Availability: PDF (request by e-mail)

Available from the Journal's Web site.
DOI: 10.1603/EC12325

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Emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) is an invasive species from Asia that was discovered in North America Canada, in 2002. Herein, we describe studies to develop an autocontamination trapping system to disseminate Beauveria bassiana to control beetle populations. The standard trap for emerald ash borer in Canada is a light green prism trap covered in an insect adhesive and baited with (Z)-3-hexenol.Wecompared of green multifunnel traps, green intercept panel traps (both with and without ßuon coating) and green prism traps for capturing emerald ash borer in a green ash plantation. The coated green multifunnel traps captured signiÞcantly more males and more females than any other trap design. We examined the efÞcacy of two native B. bassiana isolates, INRS-CFL and L49Ð1AA. In a Þeld experiment the INRS-CFL isolate attached to multifunnel traps in autocontamination chambers retained its pathogenicity to emerald ash borer adults for up to 43 d of outdoor exposure. Conidia germination of the INRS-CFL isolate was >69% after outdoor exposure in the traps for up to 57 d. The L49Ð1AA isolate was not pathogenic in simulated trap exposures and the germination rate was extremely low (<5.3%). Mean (±SEM) conidia loads on ash borer adults after being autocontaminated in the laboratory using pouches that had been exposed in traps out of doors for 29 d were 579,200 (±86,181) and 2,400 (±681) for the INRS-CFL and the L49-1AA isolates, respectively. We also examined the fungal dissemination process under field conditions using the L49-1AA isolate in a green ash plantation. Beetles were lured to baited green multifunnel traps with attached autocontamination chambers. Beetles acquired fungal conidia from cultures growing on pouches in the chambers and were recaptured on Pestick-coated traps. In total, 2,532 beetles were captured of which 165 (6.5%) had fungal growth that resembled B. bassiana. Of these 25 beetles were positive for the L49-1AA isolate.