Canadian Forest Service Publications
Development of Malacosoma disstria cytoplasmic polyhedrosis virus in Bombyx mori ovarian and tracheal tissue cultures. 1970. Sohi, S.S.; Bird, F.T.; Hayashi, Y. pages 340-351 in Proceedings IVth International Colloquium on Insect Pathology, College Park,MD, August 1970.
Issued by: Great Lakes Forestry Centre
Catalog ID: 34333
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Ovarian and tracheal tissue cultures of Bombyx marl were successfully infected with a cytoplasmic polyhedrosis virus (CPV) from Malacosoma disstria. The inoculum was free virions isolated from infected guts using sucrose density gradients. Large masses of electron•dense viroplasm containing complete and incomplete virions appeared in the cytoplasm of infected cells. Proteinaceous material accumulated to make the crystalline matrix which occluded the virions and formed the polyhedron. As polyhedra matured they attained an angular shape and a smoother outline. Crystalline inclusions and spherical bodies were observed in the nuclei of some infected cells. The shape and size of the intranuclear crystalline inclusions were the same as those of cytoplasmic polyhedra, but the spherical bodies were much smaller. No virions were seen in the intranuclear inclusions.