Canadian Forest Service Publications
Isolation of a cystoplasmic-polyhedrosis virus of the silkworm, Bombyx mori. 1970. Hayashi, Y.; Kawarabata, T.; Bird, F.T. Journal of Invertebrate Pathology 16:378-384.
Issued by: Great Lakes Forestry Centre
Catalog ID: 34346
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Larvae of the silkworm, Bombyx mori, infected with cytoplasmic-polyhedrosis virus were used as a source of vral material. Sucrose gradient centrafugation was used to isolate free virions from the cytoplasm as well as those liberated from polyhedra by treatment with weak alkali. In order to trace the rate of development of the virus, viral RNA was labeled with uridine3H and viral protein with 14C amino acid. The virions were resestant to deoxycholate; also to alkali at pH 11.0 at 40C.