Canadian Forest Service Publications
Inoculum concentration and time of application of Gliocladium roseum in relation to biocontrol of Botrytis cinerea in black spruce seedlings. 1996. Zhang, P.G.; Sutton, J.C.; Hopkin, A.A. Canadian Journal of Forest Research. 26:360-367.
Issued by: Great Lakes Forestry Centre
Catalog ID: 34464
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Inoculum concentration and time of application of Gliocladium roseum Bainier were examined in relation to sporulation of Botrytis cinerea Pers.:Fr. in container-grown seedlings of black spruce (Picea mariana (Mill.) BSP) in greenhouses. Gliocladium roseum concentrations ranging from 102 to 108 conidia/mL water plus surfactant, applied four times at 2- to 4-week intervals starting when the seedling canopies closed, increasingly suppressed the proportion of seedlings with sporulation of B. cinerea (YI) during early and midphases of epidemics, and the proportion of shoot length with sporulation of the pathogen (YS) throughout the epidemics. Concentrations of 106 and 108 conidia/mL suppressed YI and YS as or more effectively than did chlorothalonil (1.4 g active ingredient/L water) applied at the same times. One application of G. roseum (106 conidia/mL) when the seedling canopies were closing suppressed YS as effectively as did programs of two to six applications of the antagonist, or six of chlorothalonil, all at 1- and 2-week intervals beginning at canopy closure. The various programs generally did not affect YI. Logistic and Gompertz regression models were developed that adequately described YI and YS as functions of inoculum concentration and (or) time. It is concluded that G. roseum has potential as a biological control agent against B. cinerea in production systems of black spruce seedlings.