Canadian Forest Service Publications
The use of sucrose gradients in the islolation of cytoplasmic-polyhedrsis virus particles. 1968. Hayashi, Y.; Bird, F.T. Journal of Invertebrate Pathology. 11:40-44.
Issued by: Great Lakes Forestry Centre
Catalog ID: 34466
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Cytoplasmic-polyhedrosis viruses multi ply in the cytoplasm of the midgut cells of many Lepidoptera. The virus particles, which contain RNA, are icosahedra about 68 m/A in diameter and have 12 prominent projections (Hosaka and Aizawa, 1964;Bird, 1965, 1966). Some of the virus particles become occluded by protein to form poly hedra 2 fi or more in diameter, but many re main in the free state (Bird, 1965, 1966). The sucrose gradient method was used in an attempt to isolate these free virus par ticles, as well as those released by dissolv ing the polyhedra in alkali.