Canadian Forest Service Publications
Rapid identification and detection of pathogenic fungi by padlock probes. 2013. Tsui, C.K.M.; Wang, B.; Schoen, C.D.; Hamelin, R.C. Chapter 49, pages 505-517 in V.K. Gupta et al., eds. Laboratory Protocols in Fungal Biology: Current Methods in Fungal Biology, Springer.
Available from: Laurentian Forestry Centre
Catalog ID: 35235
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Fungi are important pathogens of human diseases, as well as to agricultural crop and trees. Molecular diagnostics can detect diseases early, and improve identification accuracy and follow-up disease management. The use of padlock probe is effective to facilitate these detections and pathogen identification quickly and accurately. In this chapter we describe three diagnostic assays that utilize padlock probes in combination with various technologies for the detection of pathogenic fungi.
Plain Language Summary
The fungi kingdom includes important pathogens that cause disease in humans as well as in agricultural crops and trees. Molecular diagnostics enable early detection of these pathogens, more precise diagnoses and rapid implementation of monitoring systems.
Several approaches can be used to develop molecular diagnostic tools. One approach is based on the use of padlock probes (short DNA sequences).
This book chapter illustrates three examples of diagnostic assays developed using the padlock probe approach in combination with various technologies. The efficiency, speed and accuracy of detecting and identifying different pathogens are demonstrated.
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