Canadian Forest Service Publications

Detection of double-stranded RNA (DSRNA) and virus-like particles in a Canadian isolate of Chrondrostereum purpureum (Abstract) 1994. Shamoun, S.F.; Valverde, R.A. Page 488 (Vol. 84) in Phytopathology, Proceedings: APS annual meeting, Albuquerque, NM, Aug 6-10, 1994. Phytopathology 84:488. August 6-10, 1994, Albuquerque, NM. The Society, Portland, OR.

Year: 1994

Available from: Pacific Forestry Centre

Catalog ID: 3530

Language: English

CFS Availability: Order paper copy (free)

Abstract

Chondrostereum purpureum, is an important pathogen of orchard crops and is now under evaluation as a mycoherbicide for unwanted brush species in forestry. Isolates of the fungus from Canada, New Zealand and The Netherlands were analysed by polyacrylamide gel electrophoresis for double stranded RNA (dsRNA) content. DsRNA molecules of 1.0x106 and 1.2x106 Daltons and isometric virus-like particles (VLPs) of approximately 30 nm were found in only one isolate (PFC 2064) of the 23 isolates tested. No VLPs were found in isolates in which dsRNA was not detected. Mycelial dry weight and virulence of the representative isolates were measured. The presence of dsRNA and VLPs in the isolate PFC 2064 demonstrated reduced virulence on apple fruit, but no effects on mycelial dry weight. Electrophoretic profiles of dsRNA extracted from purifications of VLPs were identical to those of dsRNA extracted from mycelium, suggesting that dsRNA is encapsidated in virus particles. The presence of dsRNA and VLPs in C. purpureum may provide a valuable marker for genetic, epidemiological and environmental fate studies.

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