Canadian Forest Service Publications

O-coumaric acid ester, a potential early signaling molecule in Pinus pinea and Pisolithus arhizus symbiosis established in vitro. 2014. Ragonezi, C.; Teixeira, D.; Caldeira, A.T.; Martins, M.R.; Santos-Silva, C.; Ganhao, E.; Klimaszewska, K.; Zavattieri, M.A. J. Plant Interactions 9:297-305.

Year: 2014

Available from: Laurentian Forestry Centre

Catalog ID: 35469

Language: English

CFS Availability: PDF (request by e-mail)

Available from the Journal's Web site.
DOI: 10.1080/17429145.2013.831489

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Abstract

During ectomycorrhizal (ECM) establishment, biochemical signals lead to the development of complex structures in both the plant and the fungus that ultimately result in the formation of an ectomycorrhiza. The cross-talk between partners begins before physical contact. Our objective was to investigate the chemical nature of the signals during the first stages of in vitro mycorrhization of Pinus pinea with Pisolithus arhizus. For this purpose a double-phase solid-liquid medium was expressly developed for the co-culture in order to simplify the extraction and further molecules analysis. O-coumaric acid ester was identified using HPLC-UV and LC-DAD-MS on the second day of co-culture and its presence was detected for up to 10 days. These results contribute to the characterization of biochemical signals during pre-colonization involving conifer species and an ECM fungus, and demonstrate the suitability of the double-phase medium developed for the growth of both organisms and for the analysis of released chemical mediators.

Plain Language Summary

When tree seedlings establish themselves in the soil, fungi begin to develop around the roots. These fungi bond with the roots to form a complex structure: mycorrhizae. These, in turn, promote growth in trees and increase their resistance to various stress factors (disease, drought, etc.). First, there is physical contact between the root and the fungi. Then, the root and fungi emit chemical signals that result in the formation of mycorrhizae.

In this study, the researchers sought to learn more about the nature of these chemical signals during the first stages of mycorrhizal formation in stone pine. They identified coumaric acid, which is present during the first 10 days of the mycorrhizal formation.

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