Canadian Forest Service Publications

Overexpression of a western white pine PR10 protein enhances cold tolerance in transgenic Arabidopsis. 2013. Liu, J.J.; Ekramoddoullah, A.K.M.; Hawkins, B.; Shah, S. Journal of Plant Biotechnology. 114(2): 217-223.

Year: 2013

Available from: Pacific Forestry Centre

Catalog ID: 35477

Language: English

CFS Availability: PDF (request by e-mail)

Available from the Journal's Web site.
DOI: 10.1007/s11240-013-0317-z

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Abstract

The PmPR10-1.10 protein from western white pine is known to be associated with frost hardiness, and upregulated by seasonal cold acclimation and biotic and abiotic stresses. To gain insight into the molecular basis of cold hardiness, we investigated the potential physiological role of PmPR10-1.10 by gene overexpression in transgenic Arabidopsis plants.Abinary vector was constructed for PmPR10-1.10 synthesis in higher plants and transgenic Arabidopsis lines were generated by Agrobacterium-mediated transformation. Following Western protein blot analysis confirming target protein production, transgenic Arabidopsis lineswere tested for cold tolerance by electrolyte leakage analysis post treatment of different freezing temperatures. Our results demonstrate that accumulation of PmPR10-1.10 protein resulted in significantly greater freezing tolerance in transgenic plants than in wild type plants. This indicates that the transfer and selection of cold acclimation proteins like PmPR10-1.10 may be a breeding strategy for the development of freezing tolerance in conifers.

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