Canadian Forest Service Publications

Effect of some organic solvents on the cytoplasmic-polyhedrosis virus of the forest tent caterpillar, Malacosoma disstria. 1971. Richards, W.C.; Hayashi, Y. Journal of Invertebrate Pathology 17:42-47.

Year: 1971

Issued by: Great Lakes Forestry Centre

Catalog ID: 36666

Language: English

Availability: PDF (request by e-mail)

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Abstract

Effect of ether, chloroform, fluorocarbon, and butanol on the cytoplasmic-polyhedrosis virion of the forest tent caterpillar, Malacosoma disstria, labeled with uridine-3H for viral RNA, has been investigated by zonal sucrose gradient centrifugagation. Free virions from the cytoplasm of midgut cells were not affected by ether treatment at room temperature (23°C), but about 50% were disrupted at higher temperature (37°C) releasing a light component, with the characteristics of viral RNA. Occluded virions, liberated from polyhedra were fairly stable to ether treatment at 37°C. It is suggested that this difference was due to the difference in maturity of free virions in the cytoplasm and occluded virions within the polyhedra. Free virions were not affected by chloroform treatment at 23°C, but at 37°C they were completely disrupted, releasing a 148 S component and viral RNA. Fluorocarbon caused only slight damage at 23°C but at 37°C two components were released, 175 S which is presumed to be part of the core, and viral RNA. Butanol disrupted intact virions completely at both 23°C and 37°C resulting in two distinct components similar to those obtained by fluorocarbon treatment at 37°C.