Canadian Forest Service Publications

A microfuge tube technique for disrupting microquantities of fungal spores. 1979. Richards, W.C.: Takai, S. Analytical Biochemistry 100:240-243.

Year: 1979

Issued by: Great Lakes Forestry Centre

Catalog ID: 36669

Language: English

Availability: PDF (request by e-mail)

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A practical apparatus was assembled and through its use a technique was developed to disrupt microquantities of Ceratocystis ulmi spores. spores were placed in a microfuge tube with buffer and glass beads. The microfuge tube was sealed and placed inside a holding tube and then subjected to rapid vibrations over a Vortex mixer. By this technique 80–90% of the spores were disrupted and it was possible to extract a high-quality ribosomal RNA fraction from the supernatant fluid of the disrupted spore sample. The technique has also proved applicable to spores of fungi other than C. ulmi.