Canadian Forest Service Publications

Comparative analysis of lignin peroxidase and manganese peroxidae activity on coniferous and deciduous wood using ToF-SIMS. 2016. MacDonald, J.; Goacher, R.E.; Abou-Zaid, M.; Master, E.R. In Applied Microbioogy and Biochemistry 8 p.

Year: 2016

Issued by: Great Lakes Forestry Centre

Catalog ID: 36955

Language: English

Availability: PDF (request by e-mail)

Available from the Journal's Web site.
DOI: 10.1007s00253-016-7560-2

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Plain Language Summary

White-rot fungi are distinguished by their ability to efficiently degrade lignin via ligninolytic type II peroxidases, including manganese peroxidase (MnP) and lignin peroxidase (LiP). In the present study, time-of flight secondary ion mass spectrometry (ToF-SIMS) was used to evaluate lignin modification in three coniferous (softwood) and three deciduous (hardwood) preparations following treatment with LiP and MnP from white-rot fungi. Percent modification of lignin was calculated as a loss of intact methoxylated lignin over nonfunctionalized aromatic rings, which is consistent with oxidative cleavage of methoxy moieties within the lignin structure. Exposure to MnP resulted in greater modification of lignin in softwood compared to hardwood (28% vs. 18% modification of lignin); and greater modification of G-lignin compared to S-lignin within the hardwood samples (21% vs. 12%). In contrast, exposure to LiP resulted in similar percent modification of lignin in all wood samples (21% vs 22%), and of G- and S-lignin within the hardwood (22% vs. 23%). These findings suggests that MnP and LiP would be useful for delignification of softwood and hardwood, respectively, both in nature and in industrial processes such as pulp and paper or liquid biofuel production. In addition, our results further demonstrate that ToF-SIMS analysis of wood fibre surfaces is an effective means to detect enzymatic modification of lignin, and may offer advantages over UV and HPCL-MS detection of solubilized delignification products.