Canadian Forest Service Publications
Root growth of somatic plants of hybrid Pinus strobus (L.) and P. wallichiana (A. B. Jacks.) is affected by the nitrogen composition of the somatic embryo germination medium. 2018. Llebrés, MT.; Avila, C.; Cánovas, FM.; Klimaszewska, K. Trees 32: 371–381.
Available from: Laurentian Forestry Centre
Catalog ID: 39087
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Mature somatic embryos of F2 hybrid Pinus strobus × Pinus wallichiana backcrossed with P. strobus converted to plants but survival of the somatic plants was not satisfactory prompting the present study on somatic seedling root growth on germination media varying in nitrogen (N) composition. The media were modifications of Litvay’s (Litvay et al. in Plant Cell Rep 4:325–328, 1985) which included two main groups: G1, G2, G3, G4 all contained inorganic N with or without glutamine (Gln) or casein hydrolysate (CH) and G5, G6, G7 contained solely glutamine and/or CH. In addition, G8 was half-strength G1 (with organic N) and G9 was half-strength CD (Campbell and Durzan in Can J Bot 53:1652–1657, 1975) without organic N. The roots of plants growing on media containing solely organic N grew about 2.55 times longer than on those containing solely inorganic N or both inorganic and organic N. The longest roots grew on G7 supplemented with CH and on G5 with both CH and Gln. Microarray analysis of somatic plants germinated on G1 versus G7 revealed that depending on the N source the somatic plants displayed changes in the transcriptome resulting in the differential expression of a range of genes involved in essential processes for plant growth and development. Roots grown in the absence of inorganic N were capable of rapid uptake of labelled inorganic 14N during the 2 h incubation in the nutrient solution. The somatic plants from G5 medium acclimatized at the rate twice as high as those from G1 (with both inorganic and organic N) and G2 (solely inorganic N) under standard fertilization regime.
Plain Language Summary
This study helped demonstrate that hybrid pine plants (white pine x Himalayan pine) resulting from somatic embryogenesis are able to develop longer roots when they are produced on a germination gel containing solely organic nitrogen sources. In standard germination gels, nitrogen sources are both organic and inorganic, or solely inorganic.
This work also demonstrated that the survival rate of plantlets produced on germination gels containing organic nitrogen is up to twice as high as the survival rate of those produced on standard gels.
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