Canadian Forest Service Publications
Microprojectile particle effect on stable transformation of black spruce via bombardment. 2004. Tian, L.; Séguin, A. Plant Mol. Biol. Rep. 22: 199a-199f.
Issued by: Laurentian Forestry Centre
Catalog ID: 39621
Availability: PDF (request by e-mail)
Three types of microprojectile particles, 1,0-µm gold, 1.3-µm tungsten, and 1.6-µm gold, were studied for their effectiveness on genetic transformation of black spruce via bombardment with somatic embryos as the target tissue. Different particles resulted in different levels of transient expression of the GUS reporter gene; 1.0-µm gold particles produced the highest level of expression, and 1.6-µm gold particles produced the lowest level. Particle type also affected stable transformation; 1.0-µm gold particles had a 10-fold higher stable transformation efficiency than did 1.6-µm gold particles and a 2-fold higher efficiency than did 1.3-µm tungsten particles. This study indicates that microprojectile particle type and size are important in bombardment-mediated plant transformation.