Canadian Forest Service Publications

Microprojectile particle effect on stable transformation of black spruce via bombardment. 2004. Tian, L.; Séguin, A. Plant Mol. Biol. Rep. 22: 199a-199f.

Year: 2004

Issued by: Laurentian Forestry Centre

Catalog ID: 39621

Language: English

Availability: PDF (request by e-mail)

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Three types of microprojectile particles, 1,0-µm gold, 1.3-µm tungsten, and 1.6-µm gold, were studied for their effectiveness on genetic transformation of black spruce via bombardment with somatic embryos as the target tissue. Different particles resulted in different levels of transient expression of the GUS reporter gene; 1.0-µm gold particles produced the highest level of expression, and 1.6-µm gold particles produced the lowest level. Particle type also affected stable transformation; 1.0-µm gold particles had a 10-fold higher stable transformation efficiency than did 1.6-µm gold particles and a 2-fold higher efficiency than did 1.3-µm tungsten particles. This study indicates that microprojectile particle type and size are important in bombardment-mediated plant transformation.