Canadian Forest Service Publications
Random amplified microsatellites (RAMS) - a novel method for characterizing genetic variation within fungi. 1996. Hantula, J.; Dusabenyagasani, M.; Hamelin, R.C. Eur. J. For. Path. 26: 159-166.
Issued by: Laurentian Forestry Centre
Catalog ID: 39911
Availability: PDF (request by e-mail)
A novel method, Random Amplified Microsatellites (RAMS, due to the nature of amplified markers as two randomly amplified microsatellites with the intervening sequence), was applied to generate DNA markers in a variety of fungi (Armillaria cepistipes, Gremmeniella abietina, Heterobasidion annosum, Phytophthora cactorum, Phlebiopsis gigantea, and _ Stereum sanguinolentum_). It is based on the polymerase chain reaction (PCR), and uses primers containing microsatellite sequences and degenerate anchors at the 5' end. The method is highly reproducible, applicable to all tested fungal species including members of the Phycomycètes, Ascomycetes and Basidiomycetes, and allows detection of interspecific and intraspecific DNA-polymorphisms.