Canadian Forest Service Publications

Random amplified microsatellites (RAMS) - a novel method for characterizing genetic variation within fungi. 1996. Hantula, J.; Dusabenyagasani, M.; Hamelin, R.C. Eur. J. For. Path. 26: 159-166.

Year: 1998

Issued by: Laurentian Forestry Centre

Catalog ID: 39911

Language: English

Availability: PDF (request by e-mail)

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Abstract

A novel method, Random Amplified Microsatellites (RAMS, due to the nature of amplified markers as two randomly amplified microsatellites with the intervening sequence), was applied to generate DNA markers in a variety of fungi (Armillaria cepistipes, Gremmeniella abietina, Heterobasidion annosum, Phytophthora cactorum, Phlebiopsis gigantea, and _ Stereum sanguinolentum_). It is based on the polymerase chain reaction (PCR), and uses primers containing microsatellite sequences and degenerate anchors at the 5' end. The method is highly reproducible, applicable to all tested fungal species including members of the Phycomyc├Ętes, Ascomycetes and Basidiomycetes, and allows detection of interspecific and intraspecific DNA-polymorphisms.