Canadian Forest Service Publications

A method for the determination of conifer foliage protein extracted using sodium dodecyl sulphate and mercaptoethanol. 1995. Ekramoddoullah, A.K.M.; Davidson, J.J. Phytochemical analysis 6: 20-24.

Year: 1995

Issued by: Pacific Forestry Centre

Catalog ID: 4031

Language: English

Availability: Not available through the CFS (click for more information).

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Abstract

Sodium dodecyl sulphate and mercaptoethanol are commonly used for extraction and solubilization of proteins, but these substances interfere with previously reported methods for the quantitation of small amounts of protein. In order to overcome this difficulty, a method has been developed for assaying small quantities of protein by (i) spotting the protein solution on a polyvinylidene difluoride membrane, (ii) staining the membrane bound protein with Coomassie Blue, (iii) destaining, and (iv) determining the optical density of the stained membrane by laser-based scanning. The determination of proteins in conifer foliage by this method is described.