Canadian Forest Service Publications
The differentiation of Chondrostereum purpureum isolates by cycloheximide sensitivity and L-dopa activity. 1995. Shamoun, S.F.; Sela, E.T.; Ramsfield, T.D.; Micales, J.A. Page 1179 Abstracts of Presentations (no. 516) (Vol. 85 (10) ) in Phytopathology, Proceedings: 1995 APS annual meeting. August 12-16, 1995, Pittsburg, PA. American Phytopathological Society, St. Paul, MN.
Issued by: Pacific Forestry Centre
Catalog ID: 4143
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Chondrostereum purpureum is a wound pathogen of hardwood trees, and is also under evaluation as a mycoherbicide for unwanted brush species in forestry and utility rights-of-way. A biochemical test (L-DOPA staining) was used to determine the genetic identity. There was no formation of a zone of antagonism (or black line) between the confronting margins of heterokaryon Canadian isolates in the presence of L-DOPA. European and New Zealand isolates when paired with each other or with Canadian isolates produced a black line after similar treatment. Cycloheximide sensitivity was determined by radial growth on cycloheximide-amended malt agar. There was little intraspecific variation in sensitivity among Canadian, European and New Zealand isolates (LD50 0.85-1.50). These biochemical data indicate that C. purpureum isolates share common metabolic pathways. Such genetic uniformity is crucial for the registration of C. purpureum as a mycoherbicide.