Canadian Forest Service Publications

A chalcone synthase/stilbene synthase DNA probe for conifers. 1996. Baker, S.M.; White, E.E. Theoretical and Applied Genetics 92: 827-831.

Year: 1996

Issued by: Pacific Forestry Centre

Catalog ID: 4336

Language: English

Availability: Not available through the CFS (click for more information).

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A probe for chalcone synthase (CHS) was generated by PCR using chalcone synthase conserved sequences. The cloned PCR product has high similarity to both chalcone synthase and stilbene synthase sequences. The probe was used to examine the organization of chalcone synthase and stilbene synthase genes in Abies procera, Pinus lambertiana, P. monticola, Picea glauca, P. sitchensis, Pseudotsuga menziesii, Taxus brevifolia, and Thuja plicata. A large number of hybridizing bands were found in all the species except T. plicata which did not cross hybridize. The hybridization patterns are highly polymorphic between the species and are also polymorphic within several of the them.