Canadian Forest Service Publications
Colonization of leaves and twigs of Rubus parviflorus and Rubus spectabilis by endophytic fungi in a reforestation site in British Columbia (Abstract) 1996. Shamoun, S.F.; Sieber, T.N. Page 95 in Healthy Plants, Healthy Planet APS/MSA Joint Annual Meeting, July 27-31, 1996, Indianapolis, Indiana. American Phytopathological Society, Indianapolis, IN.
Available from: Pacific Forestry Centre
Catalog ID: 4600
CFS Availability: Order paper copy (free)
A study was designed to determine which native facultative biotrophic or necrotrophic fungi might be used to suppress Rubus parviflorus and Rubus spectabilis, competing with conifers in reforestation sites. Healthy leaves and twigs were collected from each of 30 individuals of both Rubus species near Jordan River, Vancouver Island, B.C., Canada. Tissue pieces from leaves, petioles, and twigs were excised, surface sterilized and incubated on 2% malt extract agar. Pure cultures were isolated and identified after incubation. All leaves of both Rubus species were colonized by endophytes; 66% of the twig samples of R. spectabilis and 40% of the twig samples of R. parviflorus were also colonized. Most endophytes were Phomopsis spp. Five of Phomopsis spp. were identified and characterized according to the morphology of the cultures and fructification structures. All Phomopsis species occurred on both Rubus species. However, one of the Phomopsis species distinctly preferred R. parviflorus whereas another one preferentially colonized R. spectabilis. The other Phomopsis species randomly colonized both Rubus spp.
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