Canadian Forest Service Publications

Genetics of Cronartium ribicola. II. Variation in the ribosomal gene cluster. 1996. White, E.C.; Foord, B.M.; Kinloch, B.B., Jr. Canadian Journal of Botany 74(3): 461-468.

Year: 1996

Issued by: Pacific Forestry Centre

Catalog ID: 4602

Language: English

Availability: Order paper copy (free)

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Abstract

The ribosomal gene repeat in Cronartium ribicola J.C. Fisch is highly variable among spore samples from British Columbia, Canada. Both restriction site variation and length variation occur. Length heterogeneity results from differences in the number of subrepeats in the intergenic spacer (IGS). The number of IGS size classes in haploid cultures is limited, but is very large and highly variable in aeciospores from single cankers. The proportions of different size classes vary among cankers on different trees, and among subsamples taken around the periphery of large old cankers. The results are consistent with the fungus having a haploid infective mycelium that produces functional pycnia that result in localized dikaryotic areas following fusion between flexuous hyphae and pycnia. Restriction site variation appears lower than has been reported in range-wide samples of endemic fungal species, consistent with the hypothesis that introduction of C. ribicola to western North America was limited and does not represent the full genetic range of the species. No particular restriction site variants or IGS size classes characterize samples from particular geographic areas. No evidence for geographic races of the fungus was obtained.