Canadian Forest Service Publications
Distribution and reproduction of Bursaphelenchus xylophilus populations in wood and bark of western North American conifers. 1996. Forge, T.A.; Sutherland, J.R. Fundamentals of Applied Nematology 19(4): 341-347.
Issued by: Pacific Forestry Centre
Catalog ID: 4689
CFS Availability: Order paper copy (free)
The growth of populations of Bursaphelenchus xylophilus in wood and bark of Abies grandis, Pinus contorta, Pseudotsuga menziesii, Tsuga heterophylla and Thuja plicata was studied. Nematodes were inoculated into 6 cm diameter x 25 cm long stem segments of each tree species and incubated in plastic bags at room temperature (22 ± 4 ÝC). Nematode populations in the wood and bark were sampled separately at 4, 8 and 16 weeks after inoculation. Mean population densities in wood (pooled over sample dates) were 233, 13, 12, 2 and 0.03 nematodes/g dry wood for P. contorta, P. menziesii, A. grandis, T. heterophylla and T. plicata, respectively. Population densities in bark of the same species were 70, 267, 88, 113, and 21 nematodes/g dry bark, respectively. Nematode population growth was also studied in finely chopped autoclaved and non-autoclaved wood and bark of each tree species, and on filter papers impregnated with ethanol extracts of P. contorta and P. menziesii sapwood. Nematode population densities were greatest in P. contorta for both the autoclaved and non-autoclaved wood. After 6 weeks, population densities in non-autoclaved wood of P. contorta, P. menziesii, A. grandis, T. heterophylla and T. plicata was 137, 46, 5, 23 and 1 nematodes/g dry wood, respectively. Nematode populations grew more rapidly on filter papers impregnated with P. contorta extract than on filter papers impregnated with P. menziesii extract.