Canadian Forest Service Publications

Characterization of Canadian isolates of Chondrostereum purpureum by protein content, API ZYM and isozyme analyses. 1996. Shamoun, S.F.; Wall, R.E. European Journal of Forest Pathology 26: 333-342.

Year: 1996

Issued by: Pacific Forestry Centre

Catalog ID: 4713

Language: English

Availability: Not available through the CFS (click for more information).

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Seventeen isolates of Chondrostereum purpureum, collected from New Brunswick, Vancouver Island, and south-eastern British Columbia, Canada, were compared to determine the degree of genetic variation among isolates from different host species and different biogeographic locations. Several characteristics, including mycelial protein content, fungal biomass, API ZYM profile (semi-quantitative assay to detect the presence of 19 different enzymatic activities in an organism), and isozyme patterns were measured in these isolates. All 17 isolates shared similar API ZYM profiles, with only minor variation observed in the level of expression of specific enzymes. Of the 13 isozyme systems examined, eight (acid phosphatase, alkaline phosphatase, b-esterase, b-glucosidase, malate dehydrogenase, peroxidase, phosphoglucomutase, and polyphenoloxidase) produced measurable activity. No qualitative differences were observed between the isolates for all of these enzymes. There appeared to be limited variation between the isolates collected from across Canada and there was no correlation between specific API ZYM, and isozyme patterns and either host plant or geographical source of the isolates.