Canadian Forest Service Publications
Genotypic variation among wild isolates of Douglas-fir tussock moth (Lepidoptera: Lymantriidae) nuclear polyhedrosis virus. 1996. Laitinen, A.M.; Otvos, I.S.; Levin, D.B. Journal of Economic Entomology 89(3): 640-647.
Issued by: Pacific Forestry Centre
Catalog ID: 4907
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Larvae of the Douglas-fir tussock moth, Orgyia pseudotsugata (McDunnough), were reared from egg masses collected at 10 locations in 6 geographic regions of British Columbia. DNA of O. pseudotsugata nuclear polyhedrosis virus (OpMNPV) extracted from these larvae and from commercial OpMNPV viral-insecticide preparations (Virtuss and TM-BioControl-1) were subjected to restriction endonuclease (REN) analysis with PstI and SalI. Southern blot analyses of the REN cleavage products revealed polymorphisms in 54.5% of samples digested with PstI and 43.3% of samples digested with SalI. Five PstI genotypes and 2 SalI genotypes were detected. Each of the five PstI REN patterns demonstrated either one or the other of the SalI REN patterns, creating 10 possible combined genotypes, 8 of which were actually detected. Rare and common genotypes were mapped to each of the 6 geographic regions.