Canadian Forest Service Publications
Differential accumulation of proteins in resistant and susceptible sugar pine (Pinus lambertiana) seedlings inoculated with the white pine blister rust fungus (Cronartium ribicola) 1998. Ekramoddoullah, A.K.M.; Tan, Y. Canadian Journal of Plant Pathology 20: 308-318.
Issued by: Pacific Forestry Centre
Catalog ID: 5198
Foliar and stem proteins from 21 resistant and 21 susceptible sugar pine seedlings inoculated with Cronartium ribicola were resolved on SDS-PAGE and two-dimensional gel electrophoresis. The protein profiles of pooled and individual samples were analyzed. SDS-PAGE analysis showed the presence of a 19-kDa protein band in infected susceptible foliage. Partial N-terminal amino acid sequence analysis revealed that this band was a mixture of histone and a Cro r I protein. The Cro r I protein was fungus-derived, while the origin of histone was not clear. Utilizing fungal-specific monoclonal antibodies, we determined that about 90% of susceptible seedlings had fungal antigens, while only one resistant seedling showed the presence of fungal antigens. This illustrated the potential use of these monoclonal antibodies as probes in screening seedlings for susceptibility. In addition, significantly greater amounts of seasonally regulated Pin l I protein was found in the foliage of resistant versus susceptible seedlings. Using an improved two-dimensional gel electrophoresis method, tissue samples were resolved into 700-780 proteins. In comparisons of pooled samples from resistant versus susceptible seedlings, computer-assisted image analysis 119 significant proteins were identified in foliage and 64 significant proteins in stems. These proteins are primary candidates for future studies on their role in this white pine blister rust pathosystem.