Canadian Forest Service Publications
Establishment and characterization of weedy Rubus tissue cultures for invitro bioassays of Fusarium avenaceum phytotoxins. 1999. Hollmann, P.J.; Shamoun, S.F.; Lee, S.P. Phytopathology 89(6) Supplement: S34.
Issued by: Pacific Forestry Centre
Catalog ID: 5249
CFS Availability: Not available through the CFS (click for more information).
Weedy Rubus species (R. parviflorus, R. spectabilis and R. strigosus) negatively impact conifer seedling establishment on forest regeneration sites in Canada. Our goal is to manage these weeds using microbial metabolities,. As a first step towards microbial-based control, we have established tissue culture bioassays for large scale screening of phytotoxins. Media conditions to induce rapidly growing and friable callus and suspension cultures from leaf discs of the 3 weedy Rubus species were similar (modified MS) except for plant growth regulators used. Fast growing callus tissue from R. parviflorus, R. spectabilis and R. strigosus was induced with 4.5 uM 2.4-D, 26.,9 uM NAA/0.45 uM zeatin and 12.4 uM picloram respectively. Culture growth was characterized and ability to detect phytotoxins was determined. Callus tissues exposed to metabolites from the potential biocontrol agent Fusarium avenaceum resulted in growth inhibition, discoloration and cell death indicating that our Rubus tissue culture bioassays are effective indicators for phytotoxins.