Canadian Forest Service Publications

Somaclonal variation in cryopreserved embryogenic clones of white spruce (Picea glauca (Moench) Voss.) 1999. DeVerno, L.L.; Park, Y.S.; Bonga, J.M.; Barrett, J.D. Plant Cell Reports 18: 948-953.

Year: 1999

Issued by: Atlantic Forestry Centre

Catalog ID: 6030

Language: English

Availability: Order paper copy (free)

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Trees were regenrated from six white spruce embryogenic clones after cryopreservation for 3 and 4 years, respectively. Genetic stability was evaluated using randomly amplified polymorphic DNA (RAPD) fingerprints. Somaclonal variation was detected in some in vitro embryogenic cultures 2 and 12 months after they were re-established following cryoperservatino but not in the corresponding regenerated trees. These results suggest that trees regenerated from cryopreserved cultures in subsequent years are primarily genetically stable in the genomic regions tested and that variation observed due to the in vitro culture process infrequently affects trees regenerated from nomally maturing and germinating somatic embryos. However, trees regenerated from somatic embryos that matured or germinated abnormally in in vitro culture exhibited altered RAPD fragment patterns.