Canadian Forest Service Publications

Constructing conifer genomic libraries: a basic guide. 1989. DeVerno, L.L.; Byrne, J.R.; Pitel, J.A.; Cheliak, W.M. Forestry Canada, Petawawa National Forestry Institute, Chalk River, Ontario. Information Report PI-X-88E. 26 p.

Year: 1989

Issued by: Atlantic Forestry Centre

Catalog ID: 6876

Language: English

Series: Information Report (Petawawa)

Availability: Not available through the CFS (click for more information).

Mark record


Procedures are given for constructing genomic DNA bacteriophage libraries from conifer tissue. Although black spruce Picea mariana (Mill.) B.S.P. tissue was used, the protocols can be applied to any plant species. Included are techniques for high molecular weight genomic DNA isolation from mature needles, seedlings, and cell suspension cultures. In addition, purification of size-fractionated genomic DNA fragments, vector DNA isolation, and preparation of vector arms are outlined. Details are provided for production of recombinant clones, in vitro packaging, amplification, and evaluation of library quality. Appendices contain methods for purification of sized fragments, media and buffer formulas, and the number of recombinants required to represent genomes of various species.